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51.
Débora Cristina Rother Marco Aurélio Pizo Tadeu Siqueira Ricardo Ribeiro Rodrigues Pedro Jordano 《PloS one》2015,10(4)
Several factors decrease plant survival throughout their lifecycles. Among them, seed dispersal limitation may play a major role by resulting in highly aggregated (contagious) seed and seedling distributions entailing increased mortality. The arrival of seeds, furthermore, may not match suitable environments for seed survival and, consequently, for seedling establishment. In this study, we investigated spatio-temporal patterns of seed and seedling distribution in contrasting microhabitats (bamboo and non-bamboo stands) from the Brazilian Atlantic Forest. Spatial distribution patterns, spatial concordance between seed rain and seedling recruitment between subsequent years in two fruiting seasons (2004–2005 and 2007–2009), and the relation between seeds and seedlings with environmental factors were examined within a spatially-explicit framework. Density and species richness of both seeds and seedlings were randomly distributed in non-bamboo stands, but showed significant clustering in bamboo stands. Seed and seedling distributions showed across-year inconsistency, suggesting a marked spatial decoupling of the seed and seedling stages. Generalized linear mixed effects models indicated that only seed density and seed species richness differed between stand types while accounting for variation in soil characteristics. Our analyses provide evidence of marked recruitment limitation as a result of the interplay between biotic and abiotic factors. Because bamboo stands promote heterogeneity in the forest, they are important components of the landscape. However, at high densities, bamboos may limit recruitment for the plant community by imposing marked discordances of seed arrival and early seedling recruitment. 相似文献
52.
Lincoln José Michalski Darren Norris Tadeu Gomes de Oliveira Fernanda Michalski 《PloS one》2015,10(5)
Vertebrates are a vital ecological component of Amazon forest biodiversity. Although vertebrates are a functionally important part of various ecosystem services they continue to be threatened by anthropogenic impacts throughout the Amazon. Here we use a standardized, regularly spaced arrangement of camera traps within 25km2 to provide a baseline assessment of vertebrate species diversity in a sustainable use protected area in the eastern Brazilian Amazon. We examined seasonal differences in the per species encounter rates (number of photos per camera trap and number of cameras with photos). Generalized linear models (GLMs) were then used to examine the influence of five variables (altitude, canopy cover, basal area, distance to nearest river and distance to nearest large river) on the number of photos per species and on functional groups. GLMs were also used to examine the relationships between large predators [Jaguar (Panthera onca) and Puma (Puma concolor)] and their prey. A total of 649 independent photos of 25 species were obtained from 1,800 camera trap days (900 each during wet and dry seasons). Only ungulates and rodents showed significant seasonal differences in the number of photos per camera. The number of photos differed between seasons for only three species (Mazama americana, Dasyprocta leporina and Myoprocta acouchy) all of which were photographed more (3 to 10 fold increase) during the wet season. Mazama americana was the only species where a significant difference was found in occupancy, with more photos in more cameras during the wet season. For most groups and species variation in the number of photos per camera was only explained weakly by the GLMs (deviance explained ranging from 10.3 to 54.4%). Terrestrial birds (Crax alector, Psophia crepitans and Tinamus major) and rodents (Cuniculus paca, Dasyprocta leporina and M. acouchy) were the notable exceptions, with our GLMs significantly explaining variation in the distribution of all species (deviance explained ranging from 21.0 to 54.5%). The group and species GLMs showed some novel ecological information from this relatively pristine area. We found no association between large cats and their potential prey. We also found that rodent and bird species were more often recorded closer to streams. As hunters gain access via rivers this finding suggests that there is currently little anthropogenic impact on the species. Our findings provide a standardized baseline for comparison with other sites and with which planned management and extractive activities can be evaluated. 相似文献
53.
A comparative analysis reveals weak relationships between ecological factors and beta diversity of stream insect metacommunities at two spatial levels 下载免费PDF全文
Florian Altermatt Salman A. Al‐Shami David G. Angeler Núria Bonada Cecilia Brand Marcos Callisto Karl Cottenie Olivier Dangles David Dudgeon Andrea Encalada Emma Göthe Mira Grönroos Neusa Hamada Dean Jacobsen Victor L. Landeiro Raphael Ligeiro Renato T. Martins María Laura Miserendino Che Salmah Md Rawi Marciel E. Rodrigues Fabio de Oliveira Roque Leonard Sandin Denes Schmera Luciano F. Sgarbi John P. Simaika Tadeu Siqueira Ross M. Thompson Colin R. Townsend 《Ecology and evolution》2015,5(6):1235-1248
The hypotheses that beta diversity should increase with decreasing latitude and increase with spatial extent of a region have rarely been tested based on a comparative analysis of multiple datasets, and no such study has focused on stream insects. We first assessed how well variability in beta diversity of stream insect metacommunities is predicted by insect group, latitude, spatial extent, altitudinal range, and dataset properties across multiple drainage basins throughout the world. Second, we assessed the relative roles of environmental and spatial factors in driving variation in assemblage composition within each drainage basin. Our analyses were based on a dataset of 95 stream insect metacommunities from 31 drainage basins distributed around the world. We used dissimilarity‐based indices to quantify beta diversity for each metacommunity and, subsequently, regressed beta diversity on insect group, latitude, spatial extent, altitudinal range, and dataset properties (e.g., number of sites and percentage of presences). Within each metacommunity, we used a combination of spatial eigenfunction analyses and partial redundancy analysis to partition variation in assemblage structure into environmental, shared, spatial, and unexplained fractions. We found that dataset properties were more important predictors of beta diversity than ecological and geographical factors across multiple drainage basins. In the within‐basin analyses, environmental and spatial variables were generally poor predictors of variation in assemblage composition. Our results revealed deviation from general biodiversity patterns because beta diversity did not show the expected decreasing trend with latitude. Our results also call for reconsideration of just how predictable stream assemblages are along ecological gradients, with implications for environmental assessment and conservation decisions. Our findings may also be applicable to other dynamic systems where predictability is low. 相似文献
54.
Luciana Haddad Alex Jones Flores Cassenote Wellington Andraus Rodrigo Bronze de Martino Neli Regina de Siqueira Ortega Jair Minoro Abe Luiz Augusto Carneiro D’Albuquerque 《PloS one》2015,10(8)
Background
Liver transplantation has received increased attention in the medical field since the 1980s following the introduction of new immunosuppressants and improved surgical techniques. Currently, transplantation is the treatment of choice for patients with end-stage liver disease, and it has been expanded for other indications. Liver transplantation outcomes depend on donor factors, operating conditions, and the disease stage of the recipient. A retrospective cohort was studied to identify mortality and graft failure rates and their associated factors. All adult liver transplants performed in the state of São Paulo, Brazil, between 2006 and 2012 were studied.Methods and Findings
A hierarchical Poisson multiple regression model was used to analyze factors related to mortality and graft failure in liver transplants. A total of 2,666 patients, 18 years or older, (1,482 males; 1,184 females) were investigated. Outcome variables included mortality and graft failure rates, which were grouped into a single binary variable called negative outcome rate. Additionally, donor clinical, laboratory, intensive care, and organ characteristics and recipient clinical data were analyzed. The mortality rate was 16.2 per 100 person-years (py) (95% CI: 15.1–17.3), and the graft failure rate was 1.8 per 100 py (95% CI: 1.5–2.2). Thus, the negative outcome rate was 18.0 per 100 py (95% CI: 16.9–19.2). The best risk model demonstrated that recipient creatinine ≥ 2.11 mg/dl [RR = 1.80 (95% CI: 1.56–2.08)], total bilirubin ≥ 2.11 mg/dl [RR = 1.48 (95% CI: 1.27–1.72)], Na+ ≥ 141.01 mg/dl [RR = 1.70 (95% CI: 1.47–1.97)], RNI ≥ 2.71 [RR = 1.64 (95% CI: 1.41–1.90)], body surface ≥ 1.98 [RR = 0.81 (95% CI: 0.68–0.97)] and donor age ≥ 54 years [RR = 1.28 (95% CI: 1.11–1.48)], male gender [RR = 1.19(95% CI: 1.03–1.37)], dobutamine use [RR = 0.54 (95% CI: 0.36–0.82)] and intubation ≥ 6 days [RR = 1.16 (95% CI: 1.10–1.34)] affected the negative outcome rate.Conclusions
The current study confirms that both donor and recipient characteristics must be considered in post-transplant outcomes and prognostic scores. Our data demonstrated that recipient characteristics have a greater impact on post-transplant outcomes than donor characteristics. This new concept makes liver transplant teams to rethink about the limits in a MELD allocation system, with many teams competing with each other. The results suggest that although we have some concerns about the donors features, the recipient factors were heaviest predictors for bad outcomes. 相似文献55.
The degree of interdependence among interacting species has major implications for our understanding of the coevolutionary process and biodiversity maintenance. However, the mutualism strength among fruiting plants and their seed dispersers remains poorly understood in tropical ecosystems. We evaluated simultaneously the effectiveness of the avian seed dispersers of the mistletoe Struthanthus flexicaulis (Loranthaceae) and the contribution of its fruits to their diets in a highland rocky savanna in southeastern Brazil. The mistletoe fruits are small lipid‐rich pseudoberries available throughout the year. Four passerine birds fed on fruits, but Elaenia cristata (Tyrannidae) was the most effective disperser, responsible for more than 96 percent of the dispersed seeds. This bird swallowed fruits whole, expelling and depositing undamaged seeds by regurgitation and bill wiping on perches. From 646 dispersed seeds, 56 percent were deposited on safe sites, thin live twigs of 38 susceptible host species. Elaenia cristata were predominantly frugivorous, feeding on typically ornithocoric fruits of at least 12 species, but also on arthropods. Although fruits represented 75 percent of the feeding bouts along the year, S. flexicaulis fruits represented only 34 percent of the E. cristata diet. Our results highlight the asymmetrical nature of this mutualistic interaction, with the mistletoe life cycle locally linked to one highly effective seed disperser that is more weakly dependent on mistletoes fruits as a food source. We suggest that merging the seed dispersal effectiveness framework with diet assessment of seed dispersers is needed to clarify the asymmetries in mutualistic pairwise interactions involving plants and their animal partners. 相似文献
56.
Jane Mello Lopes Cheila de Lima Boijink Luiz Henrique Florindo Cleo Alcantara Costa Leite Ana Lúcia Kalinin William K. Milsom Francisco Tadeu Rantin 《Journal of comparative physiology. B, Biochemical, systemic, and environmental physiology》2010,180(6):797-811
In one series of experiments, heart frequency (f
H), blood pressure (P
a), gill ventilation frequency (f
R
), ventilation amplitude (V
AMP) and total gill ventilation (V
TOT) were measured in intact jeju (Hoplerythrinus unitaeniatus) and jeju with progressive denervation of the branchial branches of cranial nerves IX (glossopharyngeal) and X (vagus) without
access to air. When these fish were submitted to graded hypoxia (water PO2 ~140, normoxia to 17 mmHg, severe hypoxia), they increased f
R
, V
AMP, V
TOT and P
a and decreased f
H. In a second series of experiments, air-breathing frequency (f
RA), measured in fish with access to the surface, increased with graded hypoxia. In both series, bilateral denervation of all
gill arches eliminated the responses to graded hypoxia. Based on the effects of internal (caudal vein, 150 μg NaCN in 0.2 mL
saline) and external (buccal) injections of NaCN (500 μg NaCN in 1.0 mL water) on f
R
, V
AMP, V
TOT, P
a and f
H we conclude that the O2 receptors involved in eliciting changes in gill ventilation and associated cardiovascular responses are present on all gill
arches and monitor the O2 levels of both inspired water and blood perfusing the gills. We also conclude that air breathing arises solely from stimulation
of branchial chemoreceptors and support the hypothesis that internal hypoxaemia is the primary drive to air breathing. 相似文献
57.
Júlia D. Moreira Luisa Knorr Marcelo Ganzella Ana Paula Thomazi Carolina G. de Souza Débora G. de Souza Carolina F. Pitta Tadeu Mello e Souza Susana Wofchuk Elaine Elisabetsky Lúcia Vinadé Marcos L.S. Perry Diogo O. Souza 《Neurochemistry international》2010,56(6-7):753-759
Essential omega-3 polyunsaturated fatty acids (ω3) are crucial to brain development and function, being relevant for behavioral performance. In the present study we examined the influence of dietary ω3 in the development of the glutamatergic system and on behavior parameters in rats. Female rats received isocaloric diets, either with ω3 (ω3 group) or a ω3 deficient diet (D group). In ontogeny experiments of their litters, hippocampal immunocontent of ionotropic NMDA and AMPA glutamatergic receptors subunits (NR2 A\B and GluR1, respectively) and the alpha isoform of the calcium-calmodulin protein kinase type II (αCaMKII) were evaluated. Additionally, hippocampal [3H]glutamate binding and uptake were assessed. Behavioral performance was evaluated when the litters were adult (60 days old), through the open-field, plus-maze, inhibitory avoidance and flinch-jump tasks. The D group showed decreased immunocontent of all proteins analyzed at 02 days of life (P2) in comparison with the ω3 group, although the difference disappeared at 21 days of life (except for αCaMKII, which content normalized at 60 days old). The same pattern was found for [3H]glutamate binding, whereas [3H]glutamate uptake was not affected. The D group also showed memory deficits in the inhibitory avoidance, increased in the exploratory pattern in open-field, and anxiety-like behavior in plus-maze. Taken together, our results suggest that dietary ω3 content is relevant for glutamatergic system development and for behavioral performance in adulthood. The putative correlation among the neurochemical and behavioral alterations caused by dietary ω3 deficiency is discussed. 相似文献
58.
Fabricio Fernandes Fontana Celso Tadeu Barbosa dos Santos Flavia Maria Esteves Ademir Rocha Geisa Ferreira Fernandes Cristiane Candida do Amaral Marcos Abel Domingues Zoilo Pires De Camargo Mario León Silva-Vergara 《Mycopathologia》2010,169(3):159-165
There is some evidence that dogs can be naturally infected by Paracoccidioides brasiliensis in endemic areas of paracoccidioidomycosis. In order to evaluate canine infection with this fungus, a survey with 149 urban
and 126 rural dogs was carried out using ELISA and intradermal tests with the gp43 antigen of P. brasiliensis in Uberaba, Minas Gerais state of Brazil. Forty-one out of 149 urban dogs were euthanatized and had their lungs, liver and
spleen removed. One slice from each viscera was processed for histopathological examination and the remaining was homogenized and then cultivated on mycobiotic agar
at room temperature and Fava-Netto medium at 35°C and observed for 12 weeks. Of urban dogs, 75 (50.3%) were small adult females,
56 (36%) were strays, while 93 (64%) had been donated to the municipal zoonosis control center. Nine (6.2%) had a positive
intradermal test without statistical differences regarding gender, race, nutritional status or origin. No colonies with microscopic
or morphology appearances resembling P. brasiliensis were isolated, nor granulomatous process or fungal structures were observed from histopathological examination. Eighty (53.6%)
of the urban dogs presented seroreactivity, without statistical differences regarding gender, race, nutritional state, origin,
or positive intradermal test. Of 126 rural dogs, 102 (80.5%) presented antibodies against gp43 antigen, and this was statistically
significant in relation to the reactivity detected in urban dogs (P = 0.0001). Thus, dogs are commonly infected with P. brasiliensis, but they probably present natural resistance to develop paracoccidioidomycosis. 相似文献
59.
Penitente AR Fernandes LG Cardoso LM Silva ME Pedrosa ML Silva AL Haibara AS Moraes MF Chianca DA 《Life sciences》2007,81(7):609-614
Several studies in the literature suggest that low-protein intake is associated with increases in sympathetic efferent activity and cardiovascular disease. Among the possible mechanisms, changes in the neurotransmission of cardiovascular reflexes have been implicated. Therefore, the present study comprised the evaluation of chemoreflex responsiveness in rats subjected to a low-protein diet during the 35 days after weaning. As a result, we observed that malnourished rats presented higher levels of baseline mean arterial pressure and heart rate and exhibited a mild increase in the pressor response to chemoreflex activation. They also exhibited a massive bradycardic response to chemoreflex activation. Interestingly, bilateral ligature of the carotid body arteries further increased baseline mean arterial pressure and heart rate in malnourished animals. The data suggest severe autonomic imbalance and/or change in the central interplay between neural and cardiovascular mechanisms. 相似文献
60.
Gelfuso EA Cunha AO Mortari MR Liberato JL Paraventi KH Beleboni RO Coutinho-Netto J Lopes NP dos Santos WF 《Life sciences》2007,80(6):566-572
The aims of the present study were to investigate the anticonvulsant activity and behavioral toxicity of FrPbAII using freely moving Wistar rats. Moreover, the effectiveness of this compound against chemical convulsants was compared to that of the inhibitor of the GABAergic uptake, nipecotic acid. Our results show that FrPbAII was effective against seizures induced by the i.c.v. injection of pilocarpine (ED(50) = 0.05 microg/animal), picrotoxin (ED(50) = 0.02 microg/animal), kainic acid (ED(50) = 0.2 microg/animal) and the systemic administration of PTZ (ED(50) = 0.03 microg/animal). The anticonvulsant effect of FrPbAII differed from that of nipecotic acid in potency, as the doses needed to block the seizures were more than 10 folds lower. Toxicity assays revealed that in the rotarod, the toxic dose of the FrPbAII is 1.33 microg/animal, and the therapeutic indexes were calculated for each convulsant. Furthermore, the spontaneous locomotor activity of treated animals was not altered when compared to control animals but differed from the animals treated with nipecotic acid. Still, FrPbAII did not induce changes in any of the behavioral parameters analyzed. Finally, when tested for cognitive impairments in the Morris water maze, the i.c.v. injection of FrPbAII did not alter escape latencies of treated animals. These findings indicate that the novel GABA uptake inhibitor is a potent anticonvulsant with mild side-effects when administered to Wistar rats. 相似文献